Lasputin Zero was in the episode "Nature Nightmare". Unlike most of the villains of the series, his objectives were not revenge based. His plan was to mutate the trees of his forest into being able to defend themselves by feeding on the humans who ventured into the forest. What he did not count on was the tree's vines slowly heading toward a nearby city to gather more humans on which to feed. This disturbed him as his plan was defensive and he did not want to harm innocent people. He too was taken by his own creation, but was saved by the spies.

He made a cameo in "Evil Jerry" and "Baddies on a Blimp".